primary antibodies to lamp3  (Cell Signaling Technology Inc)

Journal: Brain

Article Title: Dysregulation of muscle cholesterol transport in amyotrophic lateral sclerosis

doi: 10.1093/brain/awae270

Figure Lengend Snippet: Free cholesterol accumulates in the late endosome/lysosome compartment in ALS muscle . Intracellular distribution of free cholesterol on transversal slices of deltoid muscle biopsies from amyotrophic lateral sclerosis (ALS) and controls (CTRL) (representative image). Lysosomes were labelled by immunofluorescence directed against LAMP3, unesterified cholesterol was stained with filipin and nuclei were stained by propidium iodide (PI). Scale bar = 50 µm. Arrows in the left panels indicate co-localizations between the cholesterol and LAMP3 and arrows in the right panels indicate lysosome without cholesterol co-localization.

Article Snippet: Slides were incubated with mouse monoclonal anti-CD63 (LAMP3) primary antibody (1:100, H5C6, DSHB) diluted in a solution of 0.05% saponin and 10% donkey serum in PBS overnight at 4°C.

Techniques: Immunofluorescence, Staining

Journal: Cureus

Article Title: Lysosome-Associated Membrane Protein-3 (LAMP3) Expression in Oral Squamous Cell Carcinoma and Its Relationship With Clinicopathological Parameters: A Cross-Sectional Study

doi: 10.7759/cureus.69790

Figure Lengend Snippet: High-power (40×) view showing LAMP3 expression.

Article Snippet: The tissues were then blocked for endogenous peroxidase activity with pre-diluted 3% hydrogen peroxide, and incubated with 1:200 dilution of anti-LAMP3 mouse monoclonal primary antibody (anti-LAMP3, clone 16H11.2, Merck Millipore, Germany).

Techniques: Expressing

Journal: Cureus

Article Title: Lysosome-Associated Membrane Protein-3 (LAMP3) Expression in Oral Squamous Cell Carcinoma and Its Relationship With Clinicopathological Parameters: A Cross-Sectional Study

doi: 10.7759/cureus.69790

Figure Lengend Snippet: Relationship of LAMP3 expression with clinicopathological parameters in oral squamous cell carcinoma. *: p-value <0.05* = Statistically significant; **: p-value <0.001 = highly significant.

Article Snippet: The tissues were then blocked for endogenous peroxidase activity with pre-diluted 3% hydrogen peroxide, and incubated with 1:200 dilution of anti-LAMP3 mouse monoclonal primary antibody (anti-LAMP3, clone 16H11.2, Merck Millipore, Germany).

Techniques: Expressing

Journal: JPEN. Journal of parenteral and enteral nutrition

Article Title: Intralipid™ Increases Nitric Oxide Release from Human Endothelial Cells during Oxidative Stress

doi: 10.1002/jpen.1834

Figure Lengend Snippet: (A) ILP increases NO release. NO production as assessed by DAF-fluorescence doubled in ECs exposed to 1% ILP for 2 hours. Concomitant incubation with L-NAME not only prevented this increase but brought it below control levels; * P < 0.05 vs control, † P < 0.05 vs ILP. (B) ILP increases formation of endosomes: CD63/LAMP3 fluorescence with ILP shows increased number of endosomes within the cell when compared to no ILP treatment; * P < 0.05 vs control.

Article Snippet: A 30-minute incubation at 37°C with the CD63/LAMP3 primary antibody (1:50, sc-5275, Santa Cruz Biotechnologies Inc.) was followed by three washes with PBS for 5 minutes each.

Techniques: Fluorescence, Incubation